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How to Calibrate an Autoclave Sciencing
Timer Calibration. Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it. Turn on the autoclave and the stopwatch simultaneously. Repeat the process if the time and the stopwatch do not match. If the autoclave timer differs from the stopwatch time by more than 30 seconds,
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STANDARD OPERATING PROCEDURE Steam Autoclaves
Autoclave 50 to 60 minutes, at temperature and pressure of 121 Co (250 oF) and 15 psig. When cycle is finished, inspect autoclave temperature tape and visually check autoclaved bags. Bags should be left to cool for several minutes before removing from autoclave.
How To Calibrate Your Autoclave (With 11 Step Procedure)
The short answer is calibration ensures consistent results from a process. Steam sterilization efficacy is highly dependent upon actual temperature. For example, if a steam autoclave is running at 120°C for 15 minutes, the theoretical lethality of that cycle is only 82% of that of a cycle running at 122°C for the same amount of exposure time.